To evaluate the performance of software in segmenting ground-glass and solid components of subsolid nodules in pulmonary adenocarcinomas.Seventy-three pulmonary adenocarcinomas manifesting as subsolid nodules were included. Two radiologists measured the maximal axial diameter of the ground-glass components on lung windows and that of the solid components on lung and mediastinal windows. Nodules were segmented using software by applying five (-850A-A?A 1/2 HU to -650A-A?A 1/2 HU) and nine (-130A-A?A 1/2 HU to -500A-A?A 1/2 HU) attenuation thresholds. We compared the manual and software measurements of ground-glass and solid components with pathology measurements of tumour and invasive components.Segmentation of ground-glass components at a threshold of -750A-A?A 1/2 HU yielded mean differences of +0.06A-A?A 1/2 mm (p=0.83, 95% limits of agreement, 4.51 to 4.67) and -2.32A-A?A 1/2 mm (p<0.001, -8.27 to 3.63) when compared with pathology and manual measurements, respectively. For solid components, mean differences between the software (at -350A-A?A 1/2 HU) and pathology measurements and between the manual (lung and mediastinal windows) and pathology measurements were -0.12A-A?A 1/2 mm (p=0.74, -5.73 to 5.55]), 0.15A-A?A 1/2 mm (p=0.73, -6.92 to 7.22), and -1.14A-A?A 1/2 mm (p<0.001, -7.93 to 5.64), respectively.Software segmentation of ground-glass and solid components in subsolid nodules showed no significant difference with pathology. - Software can effectively segment ground-glass and solid components in subsolid nodules. - Software measurements show no significant difference with pathology measurements. - Manual measurements are more accurate on lung windows than on mediastinal windows.